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For DNA extracts from the individuals for which the data obtained by sequencing the UDG-treated libraries was not enough, we used a new library protocol that uses directional splinted ligation of Illumina P5 and P7 adaptors to convert natively single-stranded DNA and heat-denatured double-stranded DNA into sequencing libraries in a single enzymatic reaction using the fully automated version of the protocol , , Otras chicas que prestan Lesbico: Q decirle a una chica q te gusta, Agencia de escorts Bergen, Putas marroquies en Tlaxcoapan

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